Table 1.
List of ASO Primers for SNP Genotyping
| Gene and SNP ID | Primer ID | Sequence (5’- 3’) | Amplicon size (bp) | MAF (1,000 G) |
|---|---|---|---|---|
| CRYAA-rs3788059 (c.190-370G > A) | SNP1-WRP | GTTGGTCCGTTAGGGTCAATAG | 174 | A: 0.0004 |
| SNP1-MRP | GTTGGTCCGTTAGGGTCAATAA | |||
| SNP1-CFP | GTGAGAAGGAGCATGTGGAAG | |||
| CRYAB-rs2070894 (c.324 + 214G > A) | SNP3-WRP | ATCCCATCATCCCATCTAAGGAG | 185 | A: 0.26 |
| SNP3-MRP | ATCCCATCATCCCATCTAAGGAA | |||
| SNP3-CFP | ATAGTCCAGGTAGTGCTATCAGCTTT | |||
| CRYBA4-rs2071861 (c.159-256A > G) | SNP5-WRP | TGATGTTTCGGGCTGGATAA | 265 | G: 0.28 |
| SNP5-MRP | TGATGTTTCGGGCTGGATAG | |||
| SNP5-CFP | AGGGTAGAGTGTGCAGGAGGTA | |||
| CRYBB2-rs5752083 c.54 + 1112C > A | SNP7-WRP | ATGCTCTCATCAACCCTGGC | 110 | A: 0.30 |
| SNP7-MRP | ATGCTCTCATCAACCCTGGA | |||
| SNP7-CFP | GAGGTGGGAGGACTGTTTGAA | |||
| CRYBB2-rs5996863 (g.17486C > T) | SNP8-WFP | CAATTCCCTTGCCTCTGACC | 208 | C: 0.38 |
| SNP8-MFP | CAATTCCCTTGCCTCTGACT | |||
| SNP8-CRP | TCAGGGTTCTTGGCTTCTCTT |
ASO: Allele-specific oligonucleotide, SNP: Single nucleotide polymorphism, CRYAA: Crystallin alpha-A, CRYAB: Crystallin alpha-B, CRYBA4: Crystallin beta-B4, CRYBB2: Crystallin beta-B2, WRP: Wild-type reverse primer, MRP: Mutant reverse primer, WFP: Wild-type forward primer, MFP: Mutant forward primer, CFP: Common forward primer, CRP: Common reverse primer, MAF: Minor allele frequency