TABLE 2.
Determination of the low limit detection level of CA v2.0
| No. of HCV RNA copies/mla | Detection of:
|
|
|---|---|---|
| HCV RNAb (OD660) | IC | |
| 6 × 103 | Pos. (3.861) | Pos. |
| 103 | Pos. (3.556) | Pos. |
| 102 | Pos. (0.613) | Pos. |
| Pos. (1.633) | Pos. | |
| Pos. (0.498) | Pos. | |
| 30 | Neg. (0.008) | Pos. |
| Neg. (0.007) | Pos. | |
| Neg. (0.007) | Pos. | |
a
Series of diluted specimens containing known amounts of HCV RNA were obtained by diluting a calibrated serum (Accurun HCV RNA).
b
Crude readings of the optical density at 660 nm (OD660) are furnished by Cobas. Samples are positive (Pos.) with optical densities of >0.15 and negative (Neg.) below this value.