Table 1. Water sampling details.
| Site | 15 mL samples | 100 mL samples | Filtration | ||||||
|---|---|---|---|---|---|---|---|---|---|
| # field reps | # technical reps | Volume (mL) | # field reps | # technical reps | Volume (mL) | # field reps | # technical reps | Volume (mL) | |
| 1 | 5 | 60 | 75 | 2 | 24 | 150 | – | – | – |
| 2 (translocated) | 5 | 60 | 75 | 2 | 24 | 150 | – | – | – |
| 3 | 5 | 60 | 75 | 2 | 24 | 150 | – | – | – |
| 4 | 5 | 60 | 75 | 2 | 24 | 150 | – | – | – |
| 5 | 5 | 60 | 75 | 4 | 48 | 300 | 1 | 12 | 1,614,000 × 103 |
| 6 (main pop) | 5 | 60 | 75 | 3 | 36 | 225 | – | – | – |
| 7 | 5 | 60 | 75 | 4 | 48 | 300 | 1 | 12 | 1,458,000 × 103 |
| 8 | 5 | 60 | 75 | 4 | 48 | 300 | 1 | 12 | 1,805,000 × 103 |
| 9 | 5 | 60 | 75 | 4 | 48 | 300 | 1 | 12 | 1,377,000 × 103 |
| 10 | 5 | 60 | 75 | 4 | 48 | 300 | 1 | 12 | 1,145,000 × 103 |
Note:
Number of field replicates, technical qPCR replicates, and volume of water processed (mL) per eDNA capture method at each sampling site during the wet season sampling. The filtration method lacks data for some cells because it was only conducted at five key sites.