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. 2021 Sep 29;16(1):1053–1063. doi: 10.1515/biol-2021-0082

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© 2021 Shanwu Wu et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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circ_CHFR silencing abolished the impacts of ox-LDL treatment on the biological behaviors of HBMECs. (a) The knockdown efficiency of si-circ_CHFR was determined by qRT-PCR. (b) The effects between ox-LDL treatment and circ_CHFR silencing on cell cycle process were disclosed by cell cycle assay in HBMECs. (c) The effects between ox-LDL treatment and circ_CHFR knockdown on the proliferation of HBMECs were investigated by MTT assay. (d, f, and g) Western blot was employed to explain the impacts between ox-LDL treatment and circ_CHFR repression on the protein expression of Ki67, Bcl-2, Bax, cleaved PARP, COL1A2, and ACTA2 in HBMECs. (e) Cell apoptosis assay was conducted to unveil the influences between ox-LDL exposure and circ_CHFR silencing on the apoptosis of HBMECs. *P < 0.05.