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. 2017 Feb 3;96(2):352–365. doi: 10.1095/biolreprod.116.143735

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please journals.permissions@oup.com

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Figure 2. — Location of syntaxin 2 (STX2) in capacitated and noncapacitated sperm using confocal microscopy. Sperm were collected and incubated under noncapacitating (ncap, A) and capacitating (cap, B) conditions for 60 min. Sperm were fixed, permeabilized, and STX2 (green) was detected with an antibody. (A) Sperm that were incubated in noncapacitating medium displayed STX2 in a punctate pattern over much of the sperm head. (B) Sperm that were incubated in capacitating medium displayed STX2 mostly at the apical ridge of the sperm head. (C) If sperm were incubated with nonimmune IgG rather than syntaxin antibody, no staining was observed.