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. 2017 Feb 3;96(2):352–365. doi: 10.1095/biolreprod.116.143735

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please journals.permissions@oup.com

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Figure 3. — SR-SIM detects syntaxin 2 (STX2) change in distribution in sperm during capacitation. Sperm were collected and incubated under capacitating conditions for 60 min. Sperm were fixed, permeabilized, and STX2 was detected with an antibody and SR-SIM. (A) Images of sperm incubated in noncapacitating medium (ncap). A punctate pattern of STX2 was observed scattered over the sperm head in the large image as well as three insets. (A’) Schematic representation of (A). (B) Images of sperm incubated in capacitating media (cap). Puncta-containing STX2 were observed primarily at the apical ridge of the sperm head. This is observed in the large image and three insets. (B’) Schematic representation of (B).