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. 2017 Feb 3;96(2):352–365. doi: 10.1095/biolreprod.116.143735

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please journals.permissions@oup.com

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Figure 5. — The location of syntaxin 2 (STX2) in sperm incubated in albumin- or bicarbonate-free medium. (A) The pattern of STX2 localization was analyzed from the images collected at different time points (0, 10, 30, 60, or 120 min) from sperm incubated in capacitating medium (C), medium without BSA (-A), and medium without HCO₃⁻ (–H). (B) Sperm incubated in medium with no HCO₃⁻ for 0 min showed punctate STX2 localization over the sperm head in the large as well as three insets. Sperm incubated in media with no HCO₃⁻ for 60 min showed punctate labeling more abundantly at the apical ridge of the sperm head in the large image and three insets. (C) Sperm incubated in medium with no albumin for 0 min showed punctate STX2 localization over the sperm head in the large as well as three insets. Sperm incubated in media with no albumin for 60 min showed punctate labeling scattered over the sperm head in the large image and three insets, much like sperm at 0 min. A total of 16 sperm were analyzed for each treatment and time point.