Figure 9.

Syntaxin 2 (STX2) and VAMP2 do not co-localize in capacitated sperm. Sperm were incubated for 60 min in capacitating conditions and stained with antibodies and appropriate fluorescent secondary antibodies to STX2 (green) and VAMP2 (red). No co-localization was observed between STX2 and VAMP2 when images collected at either 0 min (A) or 60 min (B) of capacitation time. (A΄ and B΄) are schematic representations of (A and B). (C) Graphical representation of co-localization data obtained from the SR-SIM images. A total of 16 sperm were analyzed for each treatment and time point. There was no significant correlation in localization observed at either 0 or 60 min sperm capacitation time.