Table 1.
| Antibody | Host/TYPE | Concentration/Assay | Source |
|---|---|---|---|
| STX2 (Syntaxin2) | Rabbit/ Polyclonal | 1 μg/ml/IF, 0.2 μg/ml/WB | Synaptic Systems, Goettingen, Germany |
| VAMP2 (Vesicle associated membrane protein2) | Mouse/Monoclonal | 1 μg/ml/IF | Synaptic Systems, Goettingen, Germany |
| ZP3R (Zona pellucida 3 receptor) | Mouse/Monoclonal | 1 μg/ml/IF | Pierce Thermo Scientific, Waltham, MA |
| Normal IgG, Control | Rabbit/ Polyclonal | 1 μg/ml/IF | R&D Systems, Minneapolis, MN |
| Alexa Fluor 488, Secondary antibody | Rabbit/ Polyclonal | 0.3 μg/ml/IF | Life Technologies, Carlsbad,CA |
| Alexa Fluor 568, Secondary antibody | Mouse/ Polyclonal | 0.3 μg/ml/IF | Life Technologies, Carlsbad,CA |
| HRP, Secondary Antibody | Rabbit/ Polyclonal | 0.16 μg/ml/WB | Pierce Thermo Scientific, Waltham, MA |
| Actin | Rabbit/ Polyclonal | 2.5 μg/ml/WB | Sigma-Aldrich, St.Louis, MO |
*
IF = Immunofluorescence WB = Western Blot.