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. 2021 Sep 16;8:694219. doi: 10.3389/fcvm.2021.694219

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Copyright © 2021 Cao, Li, Yang, Ke, Chen, Li, Fan, Wu, Yuan, Wang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SLSP inhibited the autophagy and early apoptosis of H9c2 cells. (A) SLSP reduced acidic vesicular organelles in H9c2 cells pretreated with rapamycin according to acridine orange (AO) staining. Arrows were added to mark the cells with acidic vesicular organelles. (B) SLSP decreased the percentage of apoptotic H9c2 cells pretreated with rapamycin through AnnexinV/PI staining. H9c2 cells were incubated with different concentrations (12.5, 25, and 50 μg/ml) of SLSP for 48 h after being pretreated with rapamycin (50 μg/ml) for 4 h and then subjected to annexin V and propidium iodide (PI) staining. (C) Rate of apoptosis. N = 3/group.