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ELISA using biotinx-C5-Fc and F2-Fc-HRP combination we were able to measure antigen when presented in the viral form. (a) Pseudotyped virus was detected to 21 TCID50 ml−1. (b) Heat-Empigen inactivated virus was detected at 103 ffu ml−1. Absorbance values above the dotted line were not actually measured, rather the sample was first diluted then measured.
