Figure 5.

Site-specific functionalization of C5Fc with PEG-biotin using mTGase mediated transglycosylation. (a) Schematic of the reaction. (b) The C5Fc nanobody was deglycosylated using PNGaseF enzyme to expose the target ‘Q’ residue and deglycosylation was monitored by mass spectrometry. (c) Trannsglutamination of C5Fc upon the reaction of amine-PEG3-biotin with the deglycosylated nanobody using microbial transglutaminase (mTGase). The addition of internal lysine residue was a completing reaction.