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. 2021 Sep 30;16(9):e0258039. doi: 10.1371/journal.pone.0258039

Fig 3. Comparison of two homogenisers for DNA extraction.

Fig 3

The FastPrep-24™ 5G (FP) and Mini-Beadbeater-24 (BB) were used in conjunction with the Isolate II Fecal Kit to detect S. stercoralis (Ss) larvae and A. lumbricoides (Al) extracted from human stool by qPCR. Stool was equally divided into 8 aliquots and extraction was performed on either the FP or BB. Four different cycling times were tested using each homogeniser and the STH1 qPCR was performed in triplicate to ascertain mean Cqs and standard deviations. *p<0.05; **p<0.005 using paired t tests.