Fig 2. CXCR3-mediated recruitment of CD4⁺ T cells is critical for iNOS production and parasite control.

(A-D) Chronic (2^o) or naïve (1^o) AMC mice were injected i.p. with 0.5mg anti-IFN-γ, 0.25mg anti-CXCR3, or 0.5mg HRPN control 2 hours prior to challenge as described in Fig 1A–1F. (B) Absolute # of the indicated CD90.2⁺CD4⁺ T cell populations per ear. (C) Frequencies of the indicated phagocyte populations. (D) Parasite loads in individual ears as determined by LDA. Each data point represents one mouse, n = 6–7 total mice pooled from two independent experiments except for the right panel in (C) %CD40⁺, n = 3–4 mice/group/experiment, representative of 2 repeat experiments. Statistical analysis was performed using one-way ANOVA with Sidaks post-test. Non-significant differences are not indicated.