Fig 3. Circulating T_EFF cell effector function at the dermal site of re-challenge occurs independently of the tissue environment in chronic or naïve mice.

(A) Chronically infected mice (2^o) were surgically conjoined to naïve AMCs (1^o) (2^o + 1^o), or naïve AMCs were surgically conjoined with one another (1^o + 1^o). (B and C) Frequency of 2^o mouse-derived CD45.2⁺ expressing CD45⁺CD4⁺ T cells (B) in samples in which CXCR3 expression (C) was also determined in both the 1° or 2° partner on day 9 post-surgery. Mice were then challenged with (D-F) 2 x 10⁵ or (G-I) 5000 L. major and analysis was conducted day 4 p.ch. (D-F) or day 14 p.ch. (G-I). (D and G) Parasite loads in individual ears as determined by LDAs. (E and H) Absolute number of the specified CD90.2⁺CD4⁺ populations per ear. (F and I) Frequency of CD11b⁺ phagocytic cells expressing the specified markers. Each data point represents one mouse; n = 6 (B and C) or 5–10 total mice/group pooled from 3 (D-F) or 2 (G-I) independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey post-test.