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. 2021 Sep 30;17(9):e1009950. doi: 10.1371/journal.ppat.1009950

Fig 1. miR-24 targets 3’UTR of STING mRNA.

Fig 1

(A) Bioinformatic softwares (Human Target scan, miRwalk) were used to identify microRNA-24 which targets 3’UTR of human STING mRNA at position 180 to 186. (B) Comparative analysis showing conserved miR-24 binding sites in STING 3’UTR across different species. (C) Human HT1080 cells were transfected with 1- Lipofectamine only, 2- Scramble control (300 pmol), 3- miR24 (300 pmol) and STING protein level was determined 36 hours post transfection by Western blotting. (D) HT1080 cells were transfected with 1- Scramble control for antimiR or 2- antimiR-24 and STING protein level was measured by western blotting 36 hours post transfection. (E) HeLa cells were transfected with 1- Luciferase expression vector+ miR-24, 2- Luciferase STING 3’ UTR vector, 3- STING 3’ UTR luciferase vector+ Scramble control, 4- Luciferase STING 3’ UTR vector + miR-24, 5- Luciferase STING 3’ UTR vector + miR-24+ antimiR-24, 6- Luciferase STING mutated 3’UTR vector + miR-24. The secretory luciferase activity in culture supernatant was measured and normalized by measuring secretory alkaline phosphatase activity. (E: Mean ± SEM, N = 3). *P<0.05, **P<0.01, ***P<0.001.