TABLE A1.
Primers used in this study
| Name | Sequence (5′ → 3′) |
|---|---|
| Retargeting TargeTron® template DNA | |
| EBS Universal (Sigma) | CGAAATTAGAAACTTGCGTTCAGTAAC |
| clcA | |
| 0095‐534‐IBS | AAAACTCGAGATAATTATCCTTAGGACTCGCAGCTGTGCGCCCAGATAG |
| 0095‐534‐EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCGCAGCTGCTAACTTACCTTTCTTTGT |
| 0095‐534‐EBS2 | TGAACGCAAGTTTCTAATTTCGATTAGTCCTCGATAGAGGAAAGTGTCT |
| Plasmid verification and sequencing | |
| TT Forward | GCAAGAGCTTGGAACTTTGAG |
| TT Reverse | GCATTCTTGTTTAGGGTATCCC |
| TargeTron insertion verification | |
| 0095 Forward | CGACATATTTGGGTGCTAGTAC |
| 0095 Reverse | GCAATCAAAAGCCAACTCCAAA |
| Kan Probe (Rodriguez et al., 2009) | |
| Forward | TGCATGGTTACTCACCACTGC |
| Reverse | TACAACCTATTAATTTCCCCTCG |
| Intron Specific (Rodriguez et al., 2009) | |
| Forward | TAGGAGAACCTATGGGAACGAAACG |
| Reverse | ACTGTACCCCTTTGCCATGT |
| Quantitative Real Time RT‐PCR | |
| Murine β‐actin Forward | CAGCCTTCCTTCTTGGGTATG |
| Murine β‐actin Reverse | TCTTCATGGTGCTAGGAGCC |
| Murine IL‐18 Forward | TCTTGGCCCAGGAACAATGG |
| Murine IL‐18 Reverse | GGTTGTACAGTGAAGTCGGC |
| Murine IL‐6 Forward | CCAAGAGATAAGCTGGAGTCACA |
| Murine IL‐6 Reverse | AACGCACTAGGTTTGCCGAG |
| Murine IL‐12 Forward | TCTGCTGCTCCACAAGAAGG |
| Murine IL‐12 Reverse | AGGGGAACTGCTACTGCTCT |
| Murine IL‐1β Forward | GCCACCTTTTGACAGTGATGAG |
| Murine IL‐1β Reverse | GACAGCCCAGGTCAAAGGTT |
| Murine TNF‐α Forward | CACAGAAAGCATGATCCGCGACGT |
| Murine TNF‐α Reverse | CGGCAGAGAGGAGGTTGACTTTCT |
| Human β‐actin Forward | CACAGAGCCTCGCCTTTGC |
| Human β‐actin Reverse | ATATCATCATCCATGGTGAGCTGG |
| Human IL‐18 Forward | AAACCTGGAATCAGATTACTTTGG |
| Human IL‐18 Reverse | TCCGGGGTGCATTATCTCTAC |
| Human IL‐1β Forward | GCCAATCTTCATTGCTCAAGTGT |
| Human IL‐1β Reverse | GGTCGGAGATTCGTAGCTGG |
| Human TNF‐α Forward | TCAGCTTGAGGGTTTGCTAC |
| Human TNF‐α Reverse | TGCACTTTGGAGTGATCGG |