Table 1. Primary Structures, HPLC Retention Times, and Molecular Mass of hECP30 Synthetic Analogs.
| molecular
mass (Da) |
|||||
|---|---|---|---|---|---|
| entry | description | sequencea | HPLC retention time (min)b | theory | found |
| 1 | hECP30 | RPFTRAQWFAIQHISPRTIAMRAINNYRWR | 8.9 | 3757.4 | 3757.6 |
| 2 | Orn analog | OPFTOAQWFAIQHISPOTIAMOAINNYOWO | 8.4 | 3505.2 | 3505.2 |
| 3 | Dab analog | XPFTXAQWFAIQHISPXTIAMXAINNYXWX | 8.3 | 3420.9 | 3421.3 |
| 4 | Har analog | ZPFTZAQWFAIQHISPZTIAMZAINNYZWZ | 9.1 | 3841.5 | 3842.2 |
| 5 | Des[Orn1-Pro2] analog | --FTOAQWFAIQHISPOTIAMOAINNYOWO | 10.8 | 3293.9 | 3293.8 |
| 6 | [Orn,1D-Pro2] analog | OpFTOAQWFAIQHISPOTIAMOAINNYOWO | 10.9 | 3505.2 | 3505.2 |
| 7 | [D-Orn,1D-Pro2] analog | opFTOAQWFAIQHISPOTIAMOAINNYOWO | 10.8 | 3505.2 | 3505.2 |
a
O, ornithine; X, 2,4-diaminobutyric acid; Z, homoarginine; d-amino acid residues in lower case.
b
All peptides were analyzed by HPLC in a linear gradient of solvent B (acetonitrile) into solvent A (H2O) from 0 to 60% over 15 min.