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. 2021 Jul 6;10(14):e019473. doi: 10.1161/JAHA.120.019473

Figure 1. LXR agonist AZ876 attenuates ISO‐induced impairment in cardiac function.

Figure 1

A, Study design. Male 129SV mice were treated with either a control diet (CTRL) or a diet supplemented with the LXR agonist AZ876) and received one subcutaneous injection on 4 consecutive days, either with NaCl (VEH) or with isoproterenol (ISO). B, Left ventricular ejection fraction and (C) left ventricular cardiac output were assessed by echocardiography. D, Representative strain images in long‐axis view (left) and short‐axis view (right) asterisks identify papillary muscles. E, Global longitudinal strain (GLS), (F) Global circumferential strain (GCS), and (G) Global radial strain (GRS). H, E/e' ratio of transmitral flow to mitral annular velocity. I, Representative images of collagen content in cardiac cross‐sections of the 4 treatment groups. Heart samples were fixated in paraffin and stained with Picrosirus red to visualize collagen content in red. J, Quantitative analysis of collagen content in the (sub‐) endocardium (left) and epicardium (right). Data presented as mean ± SEM, n=10–12 per group, *=P<0.05, **=P<0.01 2‐way ANOVA with Bonferroni post hoc test. Ao indicates aorta; LA, left atrium; and LV, left ventricle.