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. 2021 Jul 3;10(14):e021631. doi: 10.1161/JAHA.121.021631

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© 2021 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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A, Sagittal view of the D20 pig embryo. B, Frontal view of the D20 pig heart, showing a common outflow tract (OFT) arising from the right ventricle (RV). C, Sagittal view of the day 26 (D26) pig embryo, showing relative position of the atria (A), ventricle (V), liver (Li), and mesonephric kidney (MsK). D, Frontal view of the D26 pig heart, showing separate aorta (Ao) and pulmonary artery (PA) and enlargement of all 4 heart chambers. E, Sagittal view of the day 35 (D35) pig embryo. Bar=2 mm. F, Frontal view of the D35 pig heart, showing continued rotation of the heart to mesocardia positioning. G, Sagittal view of the D42 pig embryo. H, Frontal view of the D42 pig heart. A through H, Images displayed are photographs collected by necropsy of wild‐type pig embryos ranging from D20 to D42 in development. LA indicates left atrium; LV, left ventricle; and RA, right atrium.