Figure 8. High‐dose milk fat globule‐epidermal growth factor (MFG‐E8) treatment attenuates ligation‐induced actin‐related protein 2 (Arp2) expression.

Common carotid arteries (CCAs) of wild‐type (WT) mice were ligated, and recombinant MFG‐E8 (2 μg/mL) was delivered using pluronic gel into the vessels. A and B, Representative immunofluorescence images display the expression of Arp2 (A) and Arp3 (B) in the sham‐operated CCAs, ligated CCAs, and ligated CCAs treated with high‐dose MFG‐E8 in WT mice 21 days after ligation. Lumen (L), neointima (I), and media (M) of the vessels are indicated. Bar, 50 μm. C, Protein levels of Arp2 were quantified through immunoblotting. Proteins were extracted from 10 pooled CCAs per group for Western blotting and examined using antibodies against Arp2. In the quantitative analysis, the Arp2 and actin‐related protein 3 (Arp3) levels were normalized to that of β‐tubulin. DAPI indicates 4',6‐diamidino‐2‐phenylindole.