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. 2021 May 20;10(11):e019142. doi: 10.1161/JAHA.120.019142

Figure 9. Detrimental effects of asphyxial cardiac arrest (CA)/cardiopulmonary resuscitation (CPR) on the development of lymphocytes in the thymus and bone marrow.

Figure 9

A and B, Young adult mice (n=4–6/group) were subjected to 7 minutes CA or sham surgery. On day 3 after CA, single‐cell suspensions were prepared from the thymus and bone marrow. A, Percentages of double‐positive T cells (CD4+CD8+) were dramatically reduced in the thymus on day 3 after CA. Shown are representative flow cytometry plots. B, Percentages of B progenitors (lineage−B220+CD93+) were markedly reduced in the bone marrow on day 3 after CA/CPR. Shown are representative flow cytometry plots. C and D, Aged mice (n=4/group) were subjected to 5 minutes CA or sham surgery. Analyses were performed on day 3 after CA. C, Reduced percentages of double‐positive T cells in the spleen of aged mice after CA/CPR. D, Markedly reduced percentages of B progenitors in the bone marrow on day 3 after CA/CPR. Data are presented as mean±SEM. *P<0.05. ***P<0.001. ****P<0.0001.