TABLE 1.
Protein copies per cell
| Protein | No. protein copies per cell determined by: |
|||||
|---|---|---|---|---|---|---|
| Mass spectral analysisa |
Ribosome profiling (18) |
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| APEX (87, 88) | FASP-TPAb (89) | HRM-MS (90) | Minimal medium | Rich medium | Rich medium lacking Met | |
| AccB | 3,337 (3,258) | 1,144 | 2,900 | 5,637 | 34,495 | 13,718 |
| AccC | 1,726 (1,534) | 580 | 1,000 | 1,951 | 13,890 | 4,452 |
| AccA | 1,931 (2,088) | 629 | 1,400 | 1,800 | 10,155 | 4,753 |
| AccD | 1,187 (2,012) | 581 | 1,700 | 2,475 | 8,774 | 4,611 |
a
Abbreviations: APEX, absolute protein expression; FASP-TPA, filter aided sample preparation-total protein approach; HRM-MS, hyper reaction monitoring mass spectrometry; Met, methionine. The values in parentheses are from Shigella dysenteriae, a very close relative of E. coli (88).
b
The FASP-TPA values are low due to analysis of stationary-phase cells (89).