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. 2021 Sep 30;10:e71061. doi: 10.7554/eLife.71061

Figure 10. Residues 121 and 129 in the GAP region are important for MCR- and ELMOD_E-specific functions in aperture formation.

Percentage of pollen grains with indicated number of apertures in the pollen populations from independent T1 mcr plants expressing variants of MCRpr:gMCR-YFP (A–C) or MCRpr:ELMOD_A-YFP (D–F) with residues 121 and/or 129 mutated. Number of analyzed pollen grains is indicated above the bars.

Figure 10.

Figure 10—figure supplement 1. Representative aperture phenotypes observed in T1 plants related to Figure 10.

Figure 10—figure supplement 1.

(A) Representative aperture phenotypes (all furrows) observed in T1 plants related to Figure 10A–C. (B) More diverse aperture phenotypes observed in T1 plants related to Figure 10D–F. Red box highlights the most common aperture morphologies of three normal or, sometimes, disconnected furrows observed in the T1 plants related to Figure 10D. Three round apertures were only found in the T1 plants related to Figure 10E and F. ≥4 apertures were mostly round. Apertures are indicated with arrowheads. Scale bars, 10 μm. (C) Quantification of aperture morphologies (furrows vs. round) in pollen with 3 apertures and ≥4 apertures in T1 plants expressing MCRpr:gELMOD_E-YFP with MCR-like mutations at positions 121 and 129 (related to Figure 10D–F). Total number of apertures analyzed for each aperture pattern across different T1 plants expressing the same transgenic construct is indicated. Pollen with three apertures had predominantly furrows and pollen with ≥4 apertures had mostly round apertures.