(
A) Representative aperture phenotypes (all furrows) observed in T
1 plants related to
Figure 10A–C. (
B) More diverse aperture phenotypes observed in T
1 plants related to
Figure 10D–F. Red box highlights the most common aperture morphologies of three normal or, sometimes, disconnected furrows observed in the T
1 plants related to
Figure 10D. Three round apertures were only found in the T
1 plants related to
Figure 10E and F. ≥4 apertures were mostly round. Apertures are indicated with arrowheads. Scale bars, 10 μm. (
C) Quantification of aperture morphologies (furrows vs. round) in pollen with 3 apertures and ≥4 apertures in T
1 plants expressing
MCRpr:gELMOD_E-YFP with
MCR-like mutations at positions 121 and 129 (related to
Figure 10D–F). Total number of apertures analyzed for each aperture pattern across different T
1 plants expressing the same transgenic construct is indicated. Pollen with three apertures had predominantly furrows and pollen with ≥4 apertures had mostly round apertures.