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. 2021 Sep 30;10:e71061. doi: 10.7554/eLife.71061

Figure 4. ELMOD_A is involved in aperture formation.

Figure 4.

(A) Diagram of the ELMOD_A gene (At3g60260) and the CRISPR/Cas9-induced elmod_a mutation. Nucleotide and amino acid changes are indicated with red capital letters. 20 bp target sequence next to the underlined protospacer adjacent motif is shown in bold. Lowercase letters represent sequence of an intron. (B–G’) Pollen grains from elmod_a mutant and from the indicated homo- and heterozygous combinations of elmod_a and mcr mutations. (H–I’) Pollen grains from mcr elmod_a plants expressing EApr:gELMOD_A and EApr:gELMOD_A-YFP constructs. (J–K’) Pollen grains from mcr plants expressing EApr:gELMOD_A-YFP and MCRpr:gELMOD_A-YFP constructs. Apertures are indicated with arrowheads. Scale bars, 10 μm.