| Genetic reagent (Mus musculus) |
Sf1+/cre
|
Gift from Prof. Humphrey Hung-Chang Yao |
Parker lab |
Bingham et al., 2006
|
| Recombinant DNA reagent |
pRF (plasmid) |
Gift from Prof. Anne E Willis |
Willis lab |
Coldwell et al., 2000
|
| Antibody |
Anti-PRMT5 (rabbit polyclonal) |
Millipore |
Cat# 07-405 |
IF (1:200),WB (1:1000) |
| Antibody |
Anti-MEP50 (rabbit monoclonal) |
Abcam |
Cat# ab154190 |
WB (1:1000),IP (1 μg/mg protein) |
| Antibody |
Anti-WT1 (rabbit monoclonal) |
Abcam |
Cat# ab89901 |
IHC (1:400), IF (1:200), WB (1:1000) |
| Antibody |
Anti-FOXL2 (goat polyclonal) |
Abcam |
Cat# Abcam, ab5096 |
IF (1:100), WB (1:800) |
| Antibody |
Anti-HnRNPA1 (mouse monoclonal) |
Abcam |
Cat# ab5832 |
WB (1:1000)IP (1 μg/mg protein) |
| Antibody |
Anti-SYM10(rabbit polyclonal) |
Millipore |
Cat# 07-412 |
WB (1:800) |
| Antibody |
Anti-CYP11A1 (rabbit polyclonal) |
Proteintech |
Cat# 13363-1-AP |
IF (1:100), WB (1:800) |
| Antibody |
Anti-SF1 (rabbit polyclonal) |
Proteintech |
Cat# 18658-1-AP |
IF (1:200), WB (1:800) |
| Antibody |
Anti-StAR (rabbit polyclonal) |
Santa Cruz |
Cat# sc-25806 |
IF (1:100), WB (1:800) |
| Antibody |
Anti-FLAG (mouse monoclonal) |
Sigma-Aldrich |
Cat# F1804 |
WB (1:1000)RIP (1 μg/mg protein) |
| Antibody |
Anti-IgG |
Santa Cruz |
Cat# sc-2025 |
RIP (1 μg/mg protein) |
| Antibody |
Anti-3β-HSD (goat polyclonal) |
Santa Cruz |
Cat# sc-30820 |
IF (1:200), WB (1:1000) |
| Antibody |
FITC-conjugated donkey anti-goat IgG |
Jackson |
Cat# 705-095-147 |
1:150 |
| Antibody |
Cy3-conjugated donkey anti-rabbit IgG |
Jackson |
Cat# 711-165-152 |
1:300 |
| Commercial assay or kit |
Collagenase IV |
VETEC |
Cat# V900893 |
1 mg/ml |
| Commercial assay or kit |
Hyaluronidase |
Sigma Aldrich |
Cat# SIAL-H3506 |
1 mg/ml |
| Commercial assay or kit |
DNase I |
AppliChem |
Cat# A37780500 |
1 mg/ml |
| Commercial assay or kit |
EPZ015666 |
MedChemExpress |
Cat# HY-12727 |
5 μM |
| Commercial assay or kit |
RNeasy Kit |
Aidlab |
Cat# RN28 |
|
| Commercial assay or kit |
Dual luciferase reporter assay system |
Promega |
Cat# E1910 |
|
| Commercial assay or kit |
siRNA to HnRNPA1 |
ThermoFisher Scientific |
Cat# S67643, S67644 |
|
| Commercial assay or kit |
Protein A agarose beads |
GE |
Cat# 17-5280-01 |
|
| Commercial assay or kit |
Protein G agarose beads |
GE |
Cat# 17-0618-01 |
|
