Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | Charles River | Stock | |
| Genetic reagent (M. musculus) | Gad1-Cre | Gift from Dr. Diego Restrepo, recv’d frozen embryos from Tamamaki group | RRID: IMSR CARD:2065 | PMID:19915725 |
| Genetic reagent (M. musculus) | Ntsr1-Cre | Mutant Mouse Regional Resource Center | RRID: MMRRC_030648-UCD
Stock, Tg(Ntsr1-cre) GN220 Gsat/Mmucd |
PMID:17855595 |
| Genetic reagent (M. musculus) | Vgat-ires-cre knock-in (C57BL/6J) | Jackson Labs | RRID: IMSR_JAX:028862Stock, #028862 | PMID:21745644 |
| Genetic reagent (M. musculus) | Vglut2-ires-cre knock-in (C57BL/6J) | Jackson Labs | RRID: IMSR_JAX:028862Stock, #028863 | PMID:21745644 |
| Recombinant DNA reagent | AAV1.CAG.flex.GFP (virus) | Addgene | RRID: Addgene_51502Lot #: V41177 | Titer: 2.0×1013 (GFP)1.2×1013 (RFP) |
| Recombinant DNA reagent | AAV1.CAG.flex.RFP (virus) | Addgene | RRID: Addgene_28306Lot #: V5282 | |
| Recombinant DNA reagent | rAAV2.EF1a.DIO. eYFP.WPRE.pA (virus) |
UNC | RRID: Addgene_27056Lot #: AV4842F | Titer: 4.5×1012 |
| Recombinant DNA reagent | AAV8.hysn.ConFon. eYFP (virus) |
Addgene | RRID: Addgene_55650Lot #: V15284 | PMID:24908100Titer: 2.97×1013 |
| Recombinant DNA reagent | AAVretro.EF1a. FlpO (virus) |
Addgene | RRID: Addgene_55637Lot # V56725 | PMID:24908100 Titer: 1.02×1013 |
| Recombinant DNA reagent | AAV2.retro.hSyn. NLS.GFP.Cre (virus) |
Viral preparations were a gift of Dr. Jason Aoto | RRID: Addgene_175381 | PMID:23827676 |
| Recombinant DNA reagent | AAV9.EF1a.FLEX. H2B.GFP.2A.oG (virus) |
Salk Institute | RRID: Addgene_74289 | Titer: 2.41×1012 |
| Recombinant DNA reagent | AAV1.EF1a.FLEX. TVA.mCherry (virus) |
UNC | RRID: Addgene_38044 | PMID:22681690 |
| Recombinant DNA reagent | AAV1.syn.FLEX.split TVA.EGFP.tTA (virus) |
Addgene | RRID: Addgene_100798 | PMID:28847002 |
| Recombinant DNA reagent | AAV1.TREtight.mTag BFP2.B19G (virus) |
Addgene | RRID: Addgene_100799 | PMID:28847002 |
| Recombinant DNA reagent | EnvA.Gdeleted. EGFP (virus) |
Salk Institute | RRID: Addgene_32635 | |
| Recombinant DNA reagent | EnvA.Gdeleted. mCherry (virus) |
Salk Institute | RRID: Addgene_32636 | |
| Antibody | Anti-Vglut2 (Rabbit monoclonal) |
Abcam | RRID: AB_2893024Cat #: a FP1487001KT b216463 | Lot #: GR3249111-2(1:250) |
| Antibody | Anti-Gad65/67 (Rabbit polyclonal) |
Sigma-Aldrich | RRID: AB_2893025Cat#: ABN904 | Lot#: 3384833(1:200) |
| Antibody | Anti-Tyrosine Hydroxylase (Sheep polyclonal) |
MilliporeSigma | RRID: AB_90755Cat#: AB1542 | (1:200) |
| Antibody | Anti-GFP-Alexa Fluor 488 conjugate (Rabbit polyclonal) |
Invitrogen | RRID: AB_221477Cat#: A21311 | Lot #: 2017366(1:400) |
| Antibody | Anti-Rabbit DyL594 (Goat polyclonal) |
Bethyl | RRID:AB_10631380Cat#: A120-601D4 | (1:400) |
| Antibody | Anti-Mouse AF555 (Goat polyclonal) |
Life Technologies | RRID:AB_141596Cat#: A21127 | (1:400) |
| Antibody | Anti-Sheep AF 568 (Donkey polyclonal) |
Life Technologies | RRID:AB_2535753Cat#: A21099 | (1:400) |
| Commercial Assay Kit | RNAscope Intro Pack for Multiplex Fluorescent Reagent Kit v2 |
Advanced Cell Diagnostics | Cat#: 323,136 | |
| Sequence-based reagent | EYFP-C1 | Advanced Cell Diagnostics | Cat#: 312,131 | mRNA probe |
| Sequence-based reagent | Mm-Slc32a1-C2 | Advanced Cell Diagnostics | Cat#: 319,191 | mRNA probe |
| Sequence-based reagent | Mm-Slc17a6-C3 | Advanced Cell Diagnostics | Cat#: 319,171 | mRNA probe |
| Other | Opal Fluorophore Reagent Pack 520 |
Akoya Biosciences | Cat #: FP1487001KT | |
| Other | Opal Fluorophore Reagent Pack 570 |
Akoya Biosciences | Cat #: FP1488001KT | |
| Other | Opal Fluorophore Reagent Pack 690 |
Akoya Biosciences | Cat #: FP1497001KT | |
| Other | DAPI stain | Advanced Cell Diagnostics | Cat#: 323,108 |