Figure 3. ZFP91 disturbs antitumor activity of tumor-infiltrating T cells.

(A and B) Gene ontology enrichment analysis of upregulated (Up) and downregulated (Down) gene sets in tumor-infiltrating CD8⁺ T cells (A) or CD4⁺ T cells (B) from Zfp91^+/+ Cd4-Cre and Zfp91^fl/fl Cd4-Cre mice (n = 6) that were s.c. injected with MC38 murine colon cancer cells (day 14). p.adjust, adjusted P value. (C) GSEA enrichment plots of the indicated signatures in tumor-infiltrating CD8⁺ T cells from Zfp91^+/+ Cd4-Cre (WT) and Zfp91^fl/fl Cd4-Cre (KO) mice (n = 6) that were s.c. injected with MC38 murine colon cancer cells (day 14). (D) Number of OT-I cells in tumor tissues from MC38-OVA tumor–bearing mice with transferred CFSE-labeled WT OT-I and CTV-labeled KO OT-I cells (on day 7 after adoptive transfer of OT-I cells) (n = 4). (E–G) Percentage and number of IFN-γ⁺ OT-I cells (E and F) or Ki-67⁺ OT-I cells (G) in tumor tissues from MC38-OVA tumor–bearing mice with transferred OT-I cells (on day 7 after adoptive transfer of OT-I cells) (n = 4). Experiments were independently repeated 4 times (D–G). Data are presented as the mean ± SEM. **P < 0.01, by 2-tailed Student’s t test.