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PAK1 attenuates the M1 polarized macrophages. (a–f) RT-PCR analysis of expression levels of M1 macrophage markers in BMDMs by PAK1 knockdown upon LPS treatment. n = 3. (g) Western blot analysis of TNF-α and IL-6 protein level in BMDMs by PAK1 knockdown upon LPS treatment. n = 3. #P < 0.05 compared with control group. TNF-α: tumor necrosis factor-α; iNOs: inducible no synthase.
