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. 2021 Sep 23;2021:5560872. doi: 10.1155/2021/5560872

Figure 2.

Figure 2

The effect of neuraminidase on DPSC osteo-/odontoblastic differentiation. (a) FITC-PNA lectin staining of DPSCs with or without neuraminidase (100 mU/ml) treatment. (b) CCK-8 assay results of neuraminidase-treated DPSC. (c) mRNA expression of DMP1, DSPP, BSP, and RUNX2 in DPSCs treated with neuraminidase (0, 1, 10, and 100 mU/ml) for 4 days. (d) Protein expression of DSPP, DMP1, and RUNX2 in DPSCs treated with neuraminidase (0, 1, 10, and 100 mU/ml) for 4 days. (e) ALP activity and Alizarin red staining after DPSCs were treated with neuraminidase (0, 1, 10, and 100 mU/ml). Scale bars = 50 μm. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. All the experiments were repeated at least three times independently.