Figure 11.

LSNME/S^W1vaccination leads to S-induced expression of IFNγ and IL4 by CD4⁺T-cells. Splenocytes were interrogated by flow cytometry following incubation in the absence or presence of (A and B) purified, recombinant N protein or (C and D) purified, recombinant S protein, and labeling with antibodies specific for CD4 or CD8, and for IFNγ or IL4. Differences in labeling for IFNγ or IL4 in CD4⁺ CD8⁺ cell populations were plotted for (gray bars and black circles) individual six control mice, (orange bars and black squares) six mice immunized with 0.25 μg equivalents of each mRNA, and (rust bars and black triangles) six mice immunized with 4 μg equivalents of each mRNA. Height of bars represents the mean, error bars represent ± one standard error of the mean, and the statistical significance of differences between different groups is reflected in one-way ANOVA values, with ∗ denoting <0.05.