TABLE 1.
Reference yeast strains, the sizes of restriction fragments in Southern blot analysis using PHR1 and PHR2 probes, and the sizes of PCR products in experiments using C. albicans PHR1-specific primers
Species | Straina | Reference | Restriction fragment size (kb) by:
|
||||
---|---|---|---|---|---|---|---|
Southern blotting
|
PCR | ||||||
PHR1 probe
|
PHR2 probe
|
||||||
EcoRI | HindIII | EcoRI | HindIII | ||||
Candida albicans | SC5314 | Gillum et al. (5) | 3.7 | 8 | 3.9 | 1 | 1.6 |
CA019 | This study | 3.7 | 8 | NDb | ND | 1.6 | |
CA024 | This study | 3.7 | 8 | ND | ND | 1.6 | |
132A | Gallagher et al. (3) | 3.7 | 8 | ND | ND | 1.6 | |
3153 | Odds (10) | 3.7 | 8 | ND | ND | 1.6 | |
Candida stellatoidea | ATCC 11006 | 3.7 | 8 | 3.9 | 1 | 1.6 | |
ATCC 20408 | 3.7 | 8 | ND | ND | 1.6 | ||
Candida dubliniensis | CBS 7987 | 6.5 | 7 | 20 | 0.8 | NSc | |
CBS 7988 | 6.5 | 7 | 20 | 0.8 | NS | ||
CD 33 | Sullivan et al. (20) | 6.5 | 7 | ND | ND | NS | |
CD 38 | Sullivan et al. (20) | 6.5 | 7 | ND | ND | NS | |
CM 1 | Sullivan et al. (20) | 6.5 | 7 | ND | ND | NS | |
LP | This study | 6.5 | 7 | ND | ND | NS | |
Candida glabrata | ATCC 90876 | NS | NS | 12 | 7.5 | 0.7 | |
Candida tropicalis | CBS 94 | 5.5 | 14 | 7.5 | 13 | NS | |
Candida parapsilosis | CBS 604 | 11 | 13 | NS | NS | NS | |
Issatchenkia orientalis | CBS 673 | NS | NS | NS | NS | 2.1 | |
Pichia guilliermondii | CBS 566 | NS | NS | NS | NS | NS | |
Kluyveromyces marxianus | CBS 834 | NS | NS | NS | NS | 2.2 | |
Candida maltosa | CBS 5611 | NS | NS | ND | ND | 2.2 |
ATCC, American Type Culture Collection, Manassas, Va.; CBS, Centralbureau voor Schimmelcultures, Delft, The Netherlands.
ND, not determined.
NS, no signal detected.