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. 2021 Aug 19;5(10):1367–1381. doi: 10.1038/s41559-021-01525-w

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© The Author(s) 2021

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Fig. 2 — a, The relationship between expression levels (log₂(CPM)) and copy number of 45S rDNA shows extensive variation of 45S rDNA copy number and varying direction of ‘nucleolar dominance’. Grey lines connect subgenomes of the same accession. Values above the dashed line are taken as evidence for expression of a particular 45S rDNA allele, as this is above the maximum level of mis-mapping seen in the ancestral species (see Extended Data Fig. 3). b,c, FISH results of a natural A. suecica accession AS90a that has largely lost the rDNA cluster of the A.thaliana subgenome (8 copies calculated for the A. thaliana 45S rDNA and 159 copies of the A. arenosa 45S rDNA). d,e, FISH results of a natural accession ASS3 that has maintained both ancestral rDNA loci (174 copies calculated for the A. thaliana 45S rDNA and 104 copies of the A. arenosa 45S rDNA). Scale bars, 10 μm (b, d).