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. 2021 Aug 3;40(39):5799–5813. doi: 10.1038/s41388-021-01973-5

Fig. 3. miR-22 is essential for CSCs to initiate and sustain tumor growth in vivo.

Fig. 3

A The epidermis thickness of WT (n = 5) and miR-22 null mice (n = 5) were checked after a DMBA initiation and 30 d TPA promotion treatment. Scale bar, 50 µm. B Papilloma occurred on WT (n = 10) and miR-22 null mice (n = 10) dorsal skin after continuous TPA treatment. C Tumor incidence and multiplicity of WT (n = 10) and miR-22 null mice (n = 10) were shown. **p < 0.01. D cSCC occurred on WT (n = 10) and miR-22 null mice (n = 4) dorsal skin and the incidence were counted. E, F Inguinal and lung metastases in WT (n = 3) and miR-22 null mice. The arrows point to metastatic cancer. Scale bar, 50 µm. G Haematoxylin and eosin (HE) and immunofluorescence (IF) staining for ki67, p63, K14, and Lgr5 in papilloma and cSCC from WT (n = 3) and miR-22 null mice (n = 3). Scale bar, 50 µm.