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. 2021 Aug 3;40(39):5829–5842. doi: 10.1038/s41388-021-01877-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Wound-healing assay was performed to evaluate the effect of RNF141 overexpression on cell migration at 48 h after scratching (0 h). The percentage of wound closure was calculated as: (area of original wound-area of actual wound) / area of original wound × 100%. Scale bar, 500 μm. B, C Representative photos of the transwell assay showed the migrated and invasive cells of HCT116, SW480, DLD-1, and HT29 transfected with indicated lentivirus. Scale bar, 100 μm. D Tube formation assay was conducted in vitro using HUVEC cells. LV-sh-NC or LV-sh-RNF141 transfected HCT116, SW480, DLD-1, and HT29 cells were cultured in serum-free media for 24 h, and then conditioned media were collected. HUVEC cells were incubated in conditioned media for 12 h and then tube formation was photoed. Scale bar, 1 mm. Data represented the mean ± S.D. from three independent experiments, ***P < 0.001 vs indicated groups.