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. 2021 Aug 19;5(10):1382–1393. doi: 10.1038/s41559-021-01523-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Heatmap of transcript levels (TPM: transcripts per kilobase per million) of methylation pathway genes in A and T subgenomes of Arabidopsis allotetraploids, respectively. From parents (Aar/Ath) to A. suecica (Asu), the upregulated genes was marked orange, while down-regulated genes was marked blue. b, c, gBrowse tracks showing CG (green), CHG (red), and CHH (blue) methylation and mRNA expression (grey) levels in AtROS1 (b) and AaROS1–1 and AaROS1–2 (c) genes and their vicinity in A. thaliana (Ath) and A. arenosa (Aar), F₁, 733, 738 and A. suecica (Asu). The regions associated with a TE in the 5′ sequence are shown in a dashed box. The y axis scales shown above the gBrowse tracks indicate mRNA (0–30) and methylation (0–1) levels. Different letters in mRNA (TPM) indicate statistical significance of P < 0.05 (ANOVA test, n = 3).

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