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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Micron. 2021 Aug 5;150:103137. doi: 10.1016/j.micron.2021.103137

Figure 5.

Figure 5.

Binding forces to immobilized Fn as determined by AFM for (A) wild type S. aureus strain 8325 (bottom panel) and its mutants (top and middle panels) and (B) L lactis mutants. Western blots were used to quantify FnBPs in the S. aureus (C) and L. lactis (D) strains. In the insets of top panels, representative spectra from specific interactions Fn-FnBPA and Fn-FNBPB are shown in blue, whereas examples of non-specific interactions are shown gray. S. aureus wild expresses both fnbA and fnbB. Mutants of this wild-type strain express either FnBPA or FnBPB. DU5883 does not express either Fn-binding protein. L. lactis strains express exclusively FnBPA or FnBPB from S. aureus 8325. pIL253 carries an empty vector and therefore does not express FnBPA nor FnBPB.