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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Circ Res. 2021 Aug 13;129(8):e166–e182. doi: 10.1161/CIRCRESAHA.121.319104

Fig 3. atg7 OE can reverse the decline in cardiac function in the late phase of aAIC.

Fig 3.

(A) Schematics of the experimental schedule for activating atg7 in the late phase of aAIC. (B and C) High-frequency echocardiography was performed at the indicated times to quantify cardiac function. (WT, wild type; DOX, doxorubicin) (n=15). We used bright red to represent data at the early AIC phase; dark red to represent data at the late AIC phase. One-way ANOVA followed by post hoc Tukey’s test was used. (D) Evaluation of nppa and nppb gene transcript expression by quantitative RT-PCR. (n = 3). (E) Western blotting was used to assess autophagy activity in atg7;Cre fish hearts injected with DOX with or without 4HT treatment, as indicated by LC3-II expression. (F) Quantification of LC3-II and the ratio of LC3-II between the hearts treated with and without BafA1 in (E). (n = 3). Kruskal-Wallis test was used followed by post hoc Tukey’s test in (D) and (F).