Fig 8. Top2b is required for atg7-mediated autophagy in the early but not the late phase of AIC.

(A) Temporal changes of Atg7 protein level post the first dose of DOX in the mouse AIC model. N=5 mice per group. 4 wpi would be considered as the early phase, because it is equal to 1 week post the last dose of DOX. (B) H9C2 cells were transfected with the indicated siRNA. Cell lysates were analyzed by Western blots to check the knock down effects. H9C2 cells transfected with NC RNAi or Top2b RNAi were treated by DOX for 3 h (C) or 18 h (D), then levels of Atg7 and LC3II were examined by Western blots. Kruskal-Wallis test was used followed by post hoc Tukey’s test.