Figure 7. Identification of human FMRP mRNA targets.

(a) A schematic plot demonstrates the experimental procedure for eCLIP using human forebrain organoids and mouse fetal brains. (b) Pie charts display the genomic location of all human FMRP-binding sites identified from eCLIP using human forebrain organoids, mostly in CDS. (c) The number of FMRP targets and the overlap between human and mouse eCLIP genes are shown in the Venn diagram. The shared eCLIP mRNAs overlap with more than 80 percent of FMRP targets previously identified by Darnell et al. (p < 2.2e-16 using Person’s Chi-square test). (d) Shown are bar plots of gene ontology analyses using PANTHER overrepresentation test on the human-specific, the mouse-specific, and the shared FMRP targets. Numbers on the bars indicate the two-sided p-values by Fisher’s exact test and the x-axis represents fold enrichment. P values have been adjusted for multiple testing by Bonferroni correction. (e) A significant overlap between autism spectrum disorders (ASD)-related risk genes, schizophrenia (SCZ)-related risk genes and FMRP binding genes are indicated. The genes that are randomly selected did not show significant enrichment. Statistical significance was calculated by Pearson’s χ2 tests, and p-values are indicated.