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. Author manuscript; available in PMC: 2022 Feb 19.
Published in final edited form as: Nat Neurosci. 2021 Aug 19;24(10):1377–1391. doi: 10.1038/s41593-021-00913-6

Figure 8. CHD2 is a human-specific mRNA target of FMRP.

Figure 8.

(a) FMRP target genes identified by eCLIP and related to neurogenesis and synaptic plasticity showed alteration at the protein expression level. (b) Shown is the fold enrichment of CHD2 mRNA by FMRP immunoprecipitation followed by qRT-PCR in human forebrain organoids and mouse brain. Data are presented as mean ± s.e.m. (N=3, ** P=0.0024, two-tailed t test). (c) CHD2 mRNA level did not display significant alteration in D28, D56, and D84 fragile X forebrain organoids based on chi-square test. “ns” indicates that it is not statistically significant (p-value > 0.05). (d) Western blots are presented for comparing the CHD2 protein level in human organoids (Control vs FXS) and mouse embryonic brains (WT vs. Fmr1 KO) using GAPDH as loading control. Upper panel shows Western blot of CHD2 and GAPDH and the lower panel is quantification of the Western blot in human (lower left, N=3, ** P=0.0016, two-tailed t test) and mouse (lower right, N=3, NS P>0.05, two-tailed t-test). Data are presented as mean ± s.e.m. (e) Shown is overlap of the DE genes between FXS organoids and E13.5 Chd2+/− mice. Approximately half of the common DE genes (408) between human forebrain organoids and Chd2+/− mice were also verified to be bound by CHD2 in human cell (255).

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