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. 2021 Oct 1;95:153784. doi: 10.1016/j.phymed.2021.153784

Fig. 9.

Fig. 9

In vitro experiment. (A-C). Effect of different concentrations of LPS and YDJDG serum on the viability of RAW264.7 cells. * p < 0.05; ** p < 0.01 vs. control group. (D) The levels of nitric oxide in the different groups as determined by Griess reagent method. * p < 0.05; ** p < 0.01 vs. LPS group. (E) Inhibitory effects of YDJDG on LPS-induced nuclear translocation of p65 in RAW264.7 cells. Dexamethasone was used as positive control. (F) Expression of NF-κB p65, phosphorylated NF-κB, IκBα, and phosphorylated IκBα in RAW264.7 cells as determined by western blot. (G) Inhibition of macrophage activation by YDJDG in LPS-stimulated macrophages. (H) Inhibitory effect of YDJDG serum on cytokine release at different time points in LPS-treated RAW264.9 cells. **** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05 vs. LPS group. LPS, lipopolysaccharide; Dex, Dexamethasone; YDJDG, Yindan Jiedu granules.