Table 1.
Validation results of the quantitative method.
| Standard | Linear range (μg/mL) | Linear equation | R 2 | LOD (ng/mL) | LOQ (ng/mL) | Precision (RSD, %) | ||
|---|---|---|---|---|---|---|---|---|
| Intraday (low, middle, high) | Inter-day (low, middle, high) | |||||||
| 1 | Gallic acid | 0.0625-64 | y = 104867528.0426x + 63531869.8110 | 0.9974 | 0.4 | 1.0 | 2.67,1.87,2.41 | 9.14,2.23,2.42 |
| 2 | Quinic acid | 0.015625-16 | y = 233490533.2248x + 18924142.9056 | 0.9985 | 1.0 | 4.0 | 4.84,1.56,2.17 | 9.87,2.80,2.49 |
| 3 | Epicatechin | 0.015625-16 | y = 272585339.2833x + 51325033.6099 | 0.9969 | 0.2 | 0.6 | 2.62,1.42,3.37 | 8.84,4.39,2.20 |
| 4 | Hyperoside | 0.015625-8 | y = 336131315.6349x + 26185263.8044 | 0.9970 | 0.6 | 1.0 | 2.40,4.52,4.39 | 5.14,7.94,6.83 |
| 5 | Chlorogenic acid | 0.011719-12 | y = 236822254.5812x-8681388.1304 | 0.9997 | 0.8 | 2.0 | 0.90,1.48,0.99 | 6.11,2.09,2.23 |
| 6 | (-)-Gallocatechin | 0.007813-8 | y = 181374708.3263x-7335710.7997 | 0.9995 | 0.6 | 1.0 | 1.97,1.10,0.68 | 7.34,2. 13,1.88 |
| 7 | Epigallocatechin | 0.007813-8 | y = 234652428.5065x + 6820882.4056 | 0.9983 | 0.6 | 1.0 | 2.37,1.53,1.72 | 13.31,3.61,1.19 |
| 8 | Procyanidin B2 | 0.007813-8 | y = 207039382.9164x-3732613.5846 | 0.9998 | 0.6 | 1.0 | 1.88,1.31,2.10 | 5.87,2.04,2.08 |
| 9 | Neochlorogenic acid | 0.007813-8 | y = 233286007.3678x-2865114.2856 | 0.9998 | 1.0 | 4.0 | 1.65,1.25,2.10 | 5.35,1.32,2.06 |
| 10 | Epigallocatechin gallate | 0.007813-8 | y = 201974972.0708x + 1577496.8116 | 0.9979 | 0.8 | 2.0 | 1.77,2.40,3.10 | 9.27,1.61,3.27 |
| 11 | Rutin | 0.007813-8 | y = 309573862.9222x + 5346801.5931 | 0.9990 | 0.6 | 1.0 | 3.41,6.80,6.85 | 4.26,4.51,9.38 |
| 12 | trans-p-Coumaric acid | 0.03125-2 | y = 253915567.7584x + 2156877.9752 | 0.9998 | 0.6 | 2.0 | 3.38,12.22,14.65 | 6.85,15.48,11.37 |
| 13 | Quercetin | 0.007813-8 | y = 693579297.3999x + 44321253.9048 | 0.9983 | 0.6 | 1.0 | 5.74,8.09,3.08 | 10.30,4.42,7.17 |
| 14 | Kaempferol-3- O-rutinoside | 0.007813-8 | y = 317275235.2128x + 11376296.5761 | 0.9996 | 0.2 | 0.6 | 5.24,5.84,9.94 | 8.53,11.74,4.47 |
| 15 | Astragalin | 0.007813-8 | y = 440871245.5237x + 16893068.9826 | 0.9990 | 0.2 | 0.4 | 3.22,7.27,2.36 | 9.99,4.76,3.52 |
| 16 | Protocatechuic acid | 0.007813-8 | y = 133443163.8279x + 5417038.8257 | 0.9987 | 1.0 | 2.0 | 1.33,1.71,0.77 | 6.43,2.17,1.75 |
| 17 | Kaempferol | 0.003906-4 | y = 1145742735.5217x + 10566558.1343 | 0.9989 | 0.8 | 1.0 | 2.86,3.78,7.53 | 9.95,5.74,4.62 |
| 18 | (-)-Gallocatechin 3-O-gallate | 0.003906-4 | y = 247957157.35x-4760951.20 | 0.9998 | 0.8 | 1.0 | 3.82,1.50,3.41 | 6.17,4.47,3.49 |
| 19 | Vitexin | 0.003906-4 | y = 354134913.2230x + 134415.2330 | 0.9999 | 0.4 | 1.0 | 2.66,7.22,11.12 | 3.96,4.64,4.71 |
| 20 | Myricetin | 0.007813-2 | y = 595502169.4122x-12890424.3678 | 0.9994 | 0.8 | 1.0 | 2.49,4.65,4.40 | 11.86,7.45,5.88 |
| 21 | Procyanidin B1 | 0.001953-2 | y = 141557727.2076x-2136920.2090 | 0.9995 | 0.6 | 1.0 | 3.79,2.36,3.04 | 3.87,1.70,2.71 |
| 22 | Caffeic acid | 0.000977-0.25 | y = 290483148.0039x + 2032122.7323 | 0.9971 | 0.6 | 2.0 | 3.77,4.32,5.97 | 6.14,4.91,5.73 |
| 23 | Taxifolin | 0.001953-0.5 | y = 475841384.5211x-247779.2512 | 0.9997 | 0.4 | 0.6 | 5.61,4.76,3.55 | 8.39,7.22,9.29 |
| 24 | Luteolin | 0.001953-0.5 | y = 1185045545.0720x-3667626.1579 | 0.9998 | 0.4 | 1.0 | 4.44,5.49,6.16 | 14.05,7.28,5.64 |
| 25 | Apigenin | 0.000488-0.5 | y = 1504164432.8718x-879823.2686 | 0.9997 | 0.4 | 0.8 | 4.92,6.32,5.41 | 9.27,6.72,5.47 |
| 26 | Epicatechin gallate | 0.015625-8 | y = 306041466.8868x + 16782555.2605 | 0.9988 | 0.6 | 1.0 | 5.93,5.95,5.90 | 8.94,8.27,5.15 |
| 27 | Theobromine | 0.03125-32 | y = 306823878.3108x + 50508654.8548 | 0.9989 | 0.1 | 0.4 | 2.14,2.87,0.95 | 2.07,2.46,3.31 |
| 28 | Caffeine | 0.03125-32 | y = 523214802.6364x + 265990080.5366 | 0.9981 | 0.1 | 0.4 | 1.02,3.08,1.00 | 1.45,1.30,3.43 |
| 29 | L-Theanine | 0.001953-2 | y = 258253217.3246x – 7060817.7917 | 0.9978 | 0.4 | 2.0 | 0.55,2.56,3.21 | 1.62,2.95,5.19 |