TABLE 1.

Primers used for mRNA analysis.

Genes	Gene function	Forward primer	Reverse primer
GAPDH	Internal control	GTGCCGCCTGGAGAAACC	GGT​GGA​AGA​GTG​GGA​GTT​GC
FXR	BA receptor	CGG​CTG​TCA​GGA​TTT​GTG​C	GAA​GCC​CAG​GTT​GGA​ATA​GTA​AG
SHP	Form heterodimer with FXR	AACCTGCCGTCCTTCTGC	GAGCCTCAGCCACCTCGA
FGF15	Feedback inhibition of BA synthesis	GAT​CCA​CTC​TTT​CTC​TAC​GGC​TG	CGT​TCG​TTT​TGG​TCC​TCC​TC
FGFR4	FGF15 receptor	CTG​TAT​GGG​CTA​ATG​AGG​GAG​TG	TCA​GGC​GGA​GGT​CAA​GGT​AC
CYP7A1	BA synthesis enzyme	TTC​AAG​ACC​GCA​CAT​AAA​GCC	GAG​ATG​CCC​AGA​GGA​TCA​CG
CYP8B1	BA synthesis enzyme	CTC​GGG​TGT​TTC​CAA​GTG​C	GGG​CTT​CAG​GCG​ATA​GAG​G
SREBP1	Transcription of lipogenesis	AGT​CCA​GCC​TTT​GAG​GAT​AGC​C	CCG​TAG​CAT​CAG​AGG​GAG​TGA​G
ACC	Key enzyme in fatty acid synthesis	CCA​CAG​AAC​TTA​CAA​GGC​ACG	GAATTGTGAGGGTCGGCG
FASN	Key enzyme in fatty acid synthesis	GTC​AAC​AAC​CAT​AGG​CGA​TTT​C	GCA​CCC​TGA​CCC​AGA​ATA​CC
SCD1	Key enzyme in fatty acid synthesis	GAC​CTG​AAA​GCC​GAG​AAG​C	ATG​AAG​CAC​ATC​AGC​AGG​AGG
TNF-α	Inflammatory cytokine	ACG​TGG​AAC​TGG​CAG​AAG​AG	GGT​TGT​CTT​TGA​GAT​CCA​TGC
IL-6	Inflammatory cytokine	AAA​TGA​TGG​ATG​CTA​CCA​AAC​TG	CTC​TGG​CTT​TGT​CTT​TCT​TGT​TAT​C