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. 2021 Sep 30;4:1144. doi: 10.1038/s42003-021-02664-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The beam shaping optics were replaced with a telescope with cylindrical lenses (CL1, CL2) and a new focus lens (L1). The flow cell and side scatter (SSC)/fluorescence detection channel optics were kept from the original setup (Octagon). For the FSC channel, a collimating lens (L2) is followed by a spatial filter in an arrangement similar to a confocal microscope (L3, A, L4). FSC forward scatter, SSC side scatter.