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. 2021 Sep 30;11:19422. doi: 10.1038/s41598-021-97236-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Resistance of V900 to degradation by matrix metalloproteases. V900 and etanercept were incubated with recombinant human matrix metalloproteinases (MMPs) 3 and 12 for 19 or 22 h, respectively. Pre- and post-digestion samples were analysed by Western blotting alongside buffer only (no enzyme) controls. V900 was detected using a polyclonal rabbit α-SDA primary and an HRP-conjugated polyclonal swine anti-rabbit secondary antibody. Etanercept was detected using peroxidase conjugated anti-human IgG specific for Gamma-chains. Due to the high sensitivity of etanercept to MMPs, some degradation was observed in the time zero samples. Blots were visualised using an ImageQuant LAS4000 (Cytiva) on the Chemiluminescensce setting for 1 s (etanercept) or 30 s (V900). L = SuperSignal Prestained ladder. MW = Molecular weight in kDa (vertical numbers). Full length blots are shown in Supplementary Fig. S4.