Methods Table 1 –
Primers for qPCR analysis
| Primer | Sequence |
|---|---|
| AxRBL27_F | CATCAGATCAAGCAAGCAGTA |
| AxRBL27_R | CCAATGCAGCAGTTTAGATG |
| AxBetaActin_F | TCCATGAAGGCTGCCCAACT |
| AxBetaActin_R | TGGCGCCACATCTGATTGAT |
| AxGAPDH_F | GACAAGGCATCTGCTCACCT |
| AxGAPDH_R | ATGTTCTGGTTGGCACCTCT |
| AxTLR2_F | AGAGTGGCCCTGCTGTAGAA |
| AxTLR2_R | TCTCCCTGATGGAGGAACAG |
| AxTLR3_F | CCCAGAAGCAAATGCTGAAT |
| AxTLR3_R | AACAGCCTGCATGAAACTCC |
| AxTLR4_F | GGAAACACATTTGGGGACAA |
| AxTLR4_R | GGCTACAACCAGAAATGTCCA |
| AxHMGB1_F | AGAGCGACAAGCAACGCTAT |
| AxHMGB1_R | GTTGAGGCCTGTACGAGGAG |
| AxIL1B_F | CGCCGCCCACAGCAGACATCC |
| AxIL1B_R | GGGCAGGCGGCTGACTCGAA |
| AxIL4_F | CGGCAGTATGCGTCAGAGT |
| AxIL4_R | CCGTTTCAGGGTCATTTTCA |
| AxIL6_F | CAAGCAGGGATTCAGAAAGG |
| AxIL6_R | AGAGCCAGCAGGAGTTCTGT |
| AxIL8_F | CCAAGCAAATTCGGACTGTT |
| AxIL8_R | CGTCCGATGTTCCTCATTGT |
| AxIL10_F | GAACAAGGACATCAGCAACG |
| AxIL10_R | CCCCTTATCCTGCATCTTGA |
| AxIL16_F | CTCAGTCATCGGGGTCATCT |
| AxIL16_R | CACCTTCCTCCTTGTGGAGA |
| AxCOX2_F | AACTCCTCCAGCTCTTGCGCCAT |
| AxCOX2_R | TCACAAATCAGCATGCAGGCCA |
| AxTNF-a_F | GAAGACATCACCCGAAGAGAGT |
| AxTNF-a_R | GGTTCCTGGTATCGGAAATATG |
| AxSOCS3_F | CTGACCACCACCACCTCTTT |
| AxSOCS3_R | CACAGATTGGTGTCCGATTG |