Methods Table 2 –
Antibody/reagent | Clone | Application | Supplier (cat#) |
---|---|---|---|
Isolectin GS-B4 Biotin | N/A | FC (1 in 50) | Vector Labs (B-1205) |
CD18 APC | Mouse mAb TS1/18 | FC (1 in 50) | Biolegend (302114) |
CD11b PE | Rat mAB M1/70 | FC (1 in 400) | Biolegend (101208) |
p-P38 | Mouse mAB 28B10 | FC (1 in 1500) | Cell Signalling Technology (9216L) |
p-ERK | Rabbit Polyclonal | FC (1 in 200) | Cell Signalling Technology (4370S) |
Streptavidin 568 | N/A | FC (1 in 1000) | Thermo Fisher (S112226) |
Streptavidin PE-Cy7 | N/A | FC (1 in 1000) | Biolegend (405206) |
Goat anti-mouse IgG (minimal x-reactivity) PE-Cy7 | N/A | FC (1 in 1000) | Biolegend (405315) |
Donkey anti-rabbit IgG Brilliant Violet 510 | N/A | FC (1 in 300) | Biolegend (406419) |
FC: flow cytometry | |||