Table 3.
Prediction of potential miRNAs targeting PvBURPs
| miRNA_Acc | Target_Acc | Expectation | Unpaired energy | miRNA start | miRNA end | Target start | Target end |
|---|---|---|---|---|---|---|---|
| Pvu-miR156 | PvBURP3 | 5.5 | − 1 | 1 | 21 | 423 | 443 |
| Pvu-miR169 | PvBURP10 | 5.5 | − 1 | 1 | 21 | 498 | 518 |
| Pvu-miR171 | PvBURP8 | 5 | − 1 | 1 | 21 | 571 | 591 |
| Pvu-miR171 | PvBURP4 | 5.5 | − 1 | 1 | 21 | 1333 | 1354 |
| Pvu-miR319 | PvBURP2 | 6 | − 1 | 1 | 21 | 1141 | 1161 |
| Pvu-miR390 | PvBURP8 | 5 | − 1 | 1 | 21 | 717 | 737 |
| Pvu-miR390 | PvBURP10 | 6 | − 1 | 1 | 21 | 286 | 306 |
| Pvu-miR395 | PvBURP11 | 4 | − 1 | 1 | 21 | 91 | 111 |
| Pvu-miR395 | PvBURP9 | 5.5 | − 1 | 1 | 21 | 1251 | 1271 |
| Pvu-miR395 | PvBURP2 | 6 | − 1 | 1 | 21 | 98 | 118 |
| Pvu-miR395 | PvBURP11 | 4 | − 1 | 1 | 21 | 91 | 111 |
| Pvu-miR395 | PvBURP8 | 5.5 | − 1 | 1 | 21 | 585 | 605 |
| Pvu-miR397 | PvBURP2 | 5.5 | − 1 | 1 | 21 | 54 | 74 |
| Pvu-miR397 | PvBURP7 | 6 | − 1 | 1 | 21 | 21 | 41 |
| Pvu-miR408 | PvBURP3 | 6 | − 1 | 1 | 21 | 284 | 303 |
| Pvu-miR482 | PvBURP2 | 6 | − 1 | 1 | 22 | 1463 | 1484 |
| Pvu-miR1514 | PvBURP1 | 6 | − 1 | 1 | 22 | 1340 | 1361 |
| Pvu-miR4416 | PvBURP4 | 4.5 | − 1 | 1 | 21 | 924 | 944 |
| Pvu-miR4415 | PvBURP8 | 4 | − 1 | 1 | 21 | 1533 | 1553 |
| miRNA_Acc | miRNA_aligned_fragment | Alignment | Target_aligned_fragment | Inhibition |
|---|---|---|---|---|
| Pvu-miR156 | UGACAGAAGAGAGAGAGCACA | ::::.:::::::::: | AUUGGACUUGCUCUUCUCUCA | Cleavage |
| Pvu-miR169 | CAGCCAAGGGUGAUUUGCCGG | ::::::..::::::: | CACUCAAACCAUUCUUGGCUU | Cleavage |
| Pvu-miR171 | UGAUUGAGCCGUGCCAAUAUC | :.::::.:::::::::: | GGUUCUGCCGCGGCUCAAUCC | Cleavage |
| Pvu-miR171 | UUGAGCC-GCGCCAAUAUCACU | :::.::::.::::.:::: | UUUGAGGUUGGUGCCGGUUCAA | Cleavage |
| Pvu-miR319 | UGGACUGAAGGGAGCUCCUUC | ::.:::.:.:::::.:.: | GAGGGUGUUUCCUUCGCUUCU | Cleavage |
| Pvu-miR390 | AAGCUCAGGAGGGAUAGCACC | :::::::.:::::::: | UGCGCAAUCCUUCAUGAGCUA | Cleavage |
| Pvu-miR390 | AAGCUCAGGAGGGAUAGCACC | :::::.:::::::.: | CCUGCAACCUCUCCUAAGUUG | Cleavage |
| Pvu-miR395 | UGAAGUGUUUGGGGGAACUCU | :::…::::::::::: | UCUGUUUUUCCAAACACUACA | Cleavage |
| Pvu-miR395 | UGAAGUGUUUGGGGGAACUCU | :.::…:::.:.::::: | CGGCUUUUUACAAGCGCUUCA | Cleavage |
| Pvu-miR395 | CUGAAGUGUUUGGGGGAACUC | :::.:::::.::::: | CGUUUACUCCAAAGGCUUCUG | Cleavage |
| Pvu-miR395 | UGAAGUGUUUGGGGGAACUUU | :::…::::::::::: | UCUGUUUUUCCAAACACUACA | Cleavage |
| Pvu-miR395 | UUGAAGUGUUUGGAGGAACUC | :::::::.::::::: | UCAAUCCUCCAGCGACUUCAA | Cleavage |
| Pvu-miR397 | UCAUUGAGUGCAGCGUUGAUG | :::.:.:::.::..:: | ACUCACUGUUGCUUUCGGUGG | Cleavage |
| Pvu-miR397 | UCAUUGAGUGCAGCGUUGAUG | :::..:::.:::::::: | CAUUUUUGCUUUACUCAAUGU | Translation |
| Pvu-miR408 | AUGCACUGCCUCUUCCCUGGC | :::::::::.:::::: | CAAAGGGAA-AGCCGGUGCAU | Cleavage |
| Pvu-miR482 | UCUUCCCUACACCUCCCAUACC | ::.:::.::::.:::.:: | GUUGUGUGGGGUCUGUGGAGGA | Translation |
| Pvu-miR1514 | UUCAUUUUGAAAAUAGGCAUUG | :.:.:.::::::.:::.: | CGUUUUCCGUUUCCAAGAUGGA | Translation |
| Pvu-miR4416 | UACGGGUCGCUCUCACCUAGG | ::::.:::.:::.::: | CAACGGUGGGAGUGACUCGUU | Cleavage |
| Pvu-miR4415 | UUGAUUCUCAUCACAACAUGU | .:::::::.::::.::: | CAGUGUUGUGGUGAGGAGCAC | Cleavage |