Table 2.

URP and SCoT primers and their amplification results generated in the Rosa damascena Mill. germplasm

Marker	Primer	Sequence (5 → 3)	Ta (°C)	TAB	NPB	PPB	PIC	RP	MI
URP	URP-1	ATCCAGGTCCGAGACAACC	48	20	20	100	0.48	16.35	9.6
	URP-2	CCCAGCAACTGATCGCACAC	48	20	20	100	0.43	13.7	8.6
	URP-3	AGGACTCGATAACAGGCTCC	48	20	20	100	0.44	14.1	8.8
	URP-4	ATGTGTGCGATCAGTTGCTG	48	16	16	100	0.38	8.75	6.08
	URP-6	GGACAAGAAGAGGATGTGGA	48	19	19	100	0.47	14.65	8.93
	URP-8	CCTCCTCCCTCCT	48	20	20	100	0.39	11.65	7.8
	URP-9	AGGGCTGGAGGAGGGC	48	20	20	100	0.38	11.6	7.6
	URP-10	CCTGTGTGTGTGCAT	48	20	20	100	0.43	13.5	8.6
	URP-11	ATGCACACACACAGG	48	18	18	100	0.40	11.85	7.2
	URP-12	GGTGAAGCACAGGTG	48	18	18	100	0.44	12.5	7.92
	URP-13	GGTGTAGAGAGGGGT	48	19	19	100	0.45	13.6	8.55
	URP-15	GGCAGGATTGAAGC	48	20	20	100	0.43	14.35	8.6
	URP-17	AGGAGGAGGGGAAGG	48	20	20	100	0.45	14.5	9
	URP-18	GAGGGTGGCGGCTCT	48	19	19	100	0.40	11.65	7.6
	Mean			19.21	19.21	100	0.42	13.05	8.20
SCoT	SCoT-2	CAA CAA TGG CTA CCA CCC	56	17	17	100	0.38	9.9	6.46
	SCoT-3	CAA CAA TGG CTA CCA C CG	56	17	17	100	0.43	12.05	7.31
	SCoT-4	CAA CAA TGG CTA CCA CCT	54	20	20	100	0.45	14.59	9
	SCoT-5	CAA CAA TGG CTA CCA CGA	54	16	16	100	0.40	10.29	6.4
	SCoT-8	CAA CAA TGG CTA CCA CGT	54	20	20	100	0.38	11.85	7.6
	SCoT-9	CAA CAA TGG CTA CCA GCA	54	18	18	100	0.39	10.63	7.02
	SCoT-11	AAG CAA TGG CTA CCA CCA	54	18	18	100	0.41	11.61	7.38
	SCoT-12	ACG ACA TGG CGA CCA ACG	58	20	20	100	0.45	13.37	9
	SCoT-14	ACG ACA TGG CGA CCA CCG	61	17	17	100	0.43	12.15	7.31
	SCoT-15	ACG ACA TGG CGA CCG CGA	61	18	18	100	0.43	13.51	7.74
	SCoT-21	CAC CAT GGC TAC CAC CAT	56	19	19	100	0.1	13.12	1.9
	SCoT-26	ACA ATG GCT ACC ACC ATC	54	16	16	100	0.44	11.02	7.04
	Mean			18.18	18.18	100	0.37	12.09	7.35

Ta temperature annealing, TAB total amplified bands, NPB number of polymorphic, PPB percentage of polymorphism, PIC polymorphism information content, RP resolving power, MI marker index